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资源说明：A ruby API for interacting with Biomart services
= Biomart
http://github.com/dazoakley/biomart
http://rdoc.info/projects/dazoakley/biomart
Biomart provides a simple interface for working with Biomart servers
(see http://www.biomart.org for more info on Biomart itself), so you
don't have to get down and dirty with the basic webservice calls yourself.
== Install
gem install biomart
== Usage
Include the module in your code:
require "rubygems"
require "biomart"
To basically connect to a Biomart server and have access to all of its
information and meta data:
biomart = Biomart::Server.new( "http://www.biomart.org/biomart" )
# List all of the available datasets on this Biomart server
# (be patient - this will take a while on this server as there's
# a lot there...)
p biomart.list_datasets
# Grab the "kermits" dataset
kermits = biomart.datasets["kermits"]
# List it's filters and attributes
p kermits.list_filters
p kermits.list_attributes
# Count the total number of records in the dataset
p kermits.count()
# Do a count with a filter added
p kermits.count( :filters => { "sponsor" => "EUCOMM" } )
# Do a search using the default filters and attributes
# - this will return a hash with :headers (an array of the headers)
# and :data (an array of arrays of results)
#
# Doing a search like this is generally a BAD idea as biomarts tend
# to hold a LOT of data... Unless you have time to kill...
p kermits.search()
# Do a search using some specific filters (but the default attributes)
p kermits.search( :filters => { "marker_symbol" => "Cbx1" } )
# Do a search with specific filters and attributes
p kermits.search(
:filters => { "marker_symbol" => "Cbx1" },
:attributes => ["marker_symbol"]
)
# If you would like to retrieve a more useful results object - i.e. an
# array of hashes, where each hash represents a row of results (keyed
# by the attribute name), add the :process_results argument
p kermits.search(
:filters => { "marker_symbol" => "Cbx1" },
:process_results => true
)
Or if you know the dataset you wish to work with and would like to
just get on with things...
htgt_targ = Biomart::Dataset.new(
"http://www.sanger.ac.uk/htgt/biomart",
{ :name => "htgt_targ" }
)
p htgt_targ.count( :filters => { "is_eucomm" => "1" } )
# etc. etc.
See Biomart module and Class docs for more detail.
== Advanced Filtering
Most filters in biomart are just pure textual filters, i.e.
kermits.search( :filters => { "marker_symbol" => "Cbx1" } )
To filter on more than one term, simply pass a comma concatenated string,
or an array (the following two searches are equivalent):
kermits.search( :filters => { "marker_symbol" => "Cbx1,Mysm1" } )
kermits.search( :filters => { "marker_symbol" => ["Cbx1","Mysm1"] } )
Finally, there are also boolean filters (shown in the standard MartView
interface as a filter name and then two radio buttons: 'Only' or
'Excluded'). These filers can only accept the following:
- 'Only': true / 'only' / 'included'
- 'Excluded': false / 'excluded'
Here's some example usage:
snp_mart = Biomart::Dataset.new( "http://www.ensembl.org/biomart", { :name => "hsapiens_snp" } )
# These are equivalent to selecting 'Only' in MartView
snp_mart.search( :filters => { 'with_variation_annotation' => true, 'ensembl_gene' => 'ENSG00000244734' } )
snp_mart.search( :filters => { 'with_variation_annotation' => 'only', 'ensembl_gene' => 'ENSG00000244734' } )
snp_mart.search( :filters => { 'with_variation_annotation' => 'included', 'ensembl_gene' => 'ENSG00000244734' } )
# These are equivalent to selecting 'Excluded' in MartView
snp_mart.search( :filters => { 'with_variation_annotation' => false, 'ensembl_gene' => 'ENSG00000244734' } )
snp_mart.search( :filters => { 'with_variation_annotation' => 'excluded', 'ensembl_gene' => 'ENSG00000244734' } )
== Federated Searches
To perform a federated search across two datasets...
htgt = Biomart::Server.new( "http://www.sanger.ac.uk/htgt/biomart" )
res = htgt.datasets["htgt_targ"].search(
:filters => {
"status" => [
"Mice - Genotype confirmed",
"Mice - Germline transmission",
"Mice - Microinjection in progress",
"ES Cells - Targeting Confirmed"
]
},
:attributes => [
"marker_symbol",
"mgi_accession_id",
"status"
],
:federate => [
{
:dataset => htgt.datasets["mmusculus_gene_ensembl"],
:filters => {
"chromosome_name" => "1",
"start" => "1",
"end" => "10000000"
},
:attributes => []
}
]
)
The above will perform a federated query for all genes with available
mice knockout es cells in the first 10Mb of chromosome 1 from the IKMC
projects (not really important, but it's an example of a complex query).
The basic search arguments are the same as if we were searching across
a single dataset, with the addition of the :federate option, which is
an array of hashes for each dataset (and additional filters/attributes)
that we want to federate our search with.
Note: at present you can only federate across two datasets, this is
limitation in the current stable release of biomart (0.7). If you try
federate across more than two datasets, a Biomart::ArgumentError will
be raised. This limitation shall be removed from this API when it is
possible to federate across more than two datasets in biomart itself.
Count queries are only allowed on single datasets.
== Required Attributes
As biomarts are generally big de-normalised data stores, a common task
after receiving a set of results is to filter out rows that don't have
information for a given attribute or set of attributes. A facility for
doing this has been built into the gem.
htgt = Biomart::Server.new( "http://www.sanger.ac.uk/htgt/biomart" )
res = htgt.datasets["mmusculus_gene_ensembl"].search(
:filters => {
"chromosome_name" => "1",
"start" => "1",
"end" => "10000000"
},
:attributes => [
"ensembl_gene_id", "ensembl_transcript_id",
"mouse_paralog_ensembl_gene", "mouse_paralog_chromosome"
],
:required_attributes => ["mouse_paralog_ensembl_gene"]
)
The above will perform a basic search and then automatically remove
any result row that does not have a value for the "mouse_paralog_ensembl_gene"
attribute.
Note: You can specify more than one required attribute. If more than one
required attribute is specified, ALL of these attributes must be present
for a data row to be returned (it is using AND logic).
== Using a Proxy
If you need to channel all of your requests via a proxy, specify your
proxy via Biomart.proxy:
Biomart.proxy = "http://proxy.example.com/"
Now all requests made through Biomart will be proxied via proxy.example.com.
Alternatively you can also set your proxy url in the environment variable
'http_proxy', and Biomart will automatically detect this.
== Setting a Timeout Value
If you need to alter the default request timeout value, you can alter this
globally via Biomart.timeout:
Biomart.timeout = 60 # For a 60 second timeout...
Or on a per-request basis by passing the :timeout option when searching:
kermits.search(
:filters => { "marker_symbol" => "Cbx1" },
:process_results => true,
:timeout => 60
)
== Meta
Written by Darren Oakley (daz dot oakley at gmail dot com)
http://github.com/dazoakley/biomart
http://rdoc.info/projects/dazoakley/biomart
== License
(The MIT License)
Copyright (c) 2009 Darren Oakley
Permission is hereby granted, free of charge, to any person obtaining
a copy of this software and associated documentation files (the
'Software'), to deal in the Software without restriction, including
without limitation the rights to use, copy, modify, merge, publish,
distribute, sublicense, and/or sell copies of the Software, and to
permit persons to whom the Software is furnished to do so, subject to
the following conditions:
The above copyright notice and this permission notice shall be
included in all copies or substantial portions of the Software.
THE SOFTWARE IS PROVIDED 'AS IS', WITHOUT WARRANTY OF ANY KIND,
EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF
MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT.
IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY
CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT,
TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE
SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.
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